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Вы можете ознакомиться и скачать презентацию на тему SNAP i.d. Workflow Value Proposition. Доклад-сообщение содержит 21 слайдов. Презентации для любого класса можно скачать бесплатно. Если материал и наш сайт презентаций Mypresentation Вам понравились – поделитесь им с друзьями с помощью социальных кнопок и добавьте в закладки в своем браузере.

Слайды и текст этой презентации

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SNAP i.d. Workflow Value Proposition Reduces incubation times in Western Blotting Compatible with all reagents and membranes No directly competitive product on the market

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SNAP i.d. Workflow Value Proposition

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SNAP i.d. Components

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Additional Items Chemical duty pump (WP6111560, WP6122050)

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SNAP i.d. SKU’s

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SNAP i.d. Protocol Overview: Blot Holder Assembly

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SNAP i.d. Protocol Overview: Immunodetection

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Feature and Benefits Summary

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Product Position Superior Blots in a SNAP! Optimize your blots Increase the quality of your blots And do it FAST! Results for your afternoon meeting! (Customer quote)

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Product position: Optimization All blots should be optimized but most researchers don’t have the time to do this tedious process. SNAP i.d. makes optimization easy by allowing 6 blots to be processed simultaneously in only 30 minutes! What do they typically optimize? Antibody concentrations/dilutions Detection reagent conditions Target protein concentrations (more rarely) Blocking conditions (a little secret to sensitivity) SNAP i.d. permits fast optimization for better results. Point out the Immunodetection handbook Western blotting section for helpful hints.

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How it Works Traditional western blotting takes a variety of formats and reagent conditions to accomplish. It’s a passive process! SNAP i.d. actively drives reagents through the membrane to increase the quality of the blots and increase the speed of immunodetection! It’s a combination of reagent flows and concentrations

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How it Works – reagent flows

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How it Works – reagent flows Blocking Efficient coverage of membrane which yields higher sensitivity Can use 1/10th-1/100th less concentrated blocking solution to minimize overblocking Actively driven vacuum flow coats inner surfaces of membrane in 20 sec

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Compatible Blocking Reagents and Recommended Concentrations

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How it Works – reagent flows Washing Unbound Antibodies are thoroughly flushed out of the membrane Yields lower backgrounds which may yield higher Sensitivity

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Standard vs. SNAP i.d. - concentrations

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Concentrations: The Rule of 3.5 Block in 0.5% NFDM vs 1.0%-5.0% NFDM To prevent clogging of flow distributor To minimize overblocking and maximize sensitivity